Bacteriological and Molecular Detection of Porphyromonas gingivalis from periodontitis Patients

Ali A. Obais1, Maysaa S.M. Al-shukri2, Abeer Fauzi M. Al-Rubaye3
College of Medical and Health Technique/ Department of Anesthesia/ Sawa University1
Collage of Medicine/ Department of Microbiology/ Babylon University2
College of Science for women/ Department of Biology/ Babylon University3

Abstract
Objective :The present study was conducted to detect P gingivalis by culture and molecular method from
clinical oral infection sample. Method: hundred fifty (150) paper point were collected from periodontitis
patients who attended to specific dental health center and outpatient clinics of dentistry in Al-Hillah
city/ Iraq during the duration from (April 2022 to September 2022 ) . The age of patient (5– 72)years. The
samples were collected by paper point the paper point culture on blood agar and selective media(P.
gingivalis agar (P.GING)) for isolation of P gingivalis then diagnosis conform by molecular method.
Results: The result showed that out of 150 samples 15(10%) P.gingivalis isolated from this fifteen isolate
only 7(4.66%) confirmed with molecular detection as P.gingivalis. P.gingivalis isolated in high rate
6(4%) from age group (25-35) and 4(2.66) in the age group (15-25) while only 2 (1.33%) isolate obtained
from the age group (45-55) and 3 (2%) isolate from age group (55-70). Conclusion: quantitative
evaluation, RT-PCR offers the advantage of eliminating false positives which could otherwise play
abysmal role in conventional detection techniques.
Key words: Periodontist, Real Time –PCR , P.gingivalis .

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