Saba Hameed Alshebani1 , Ameer Abed Kadhim Hamid2
1,2Department of Basic Sciences, college 0f Dentistry, University of Al-Qadisiya

Abstract:
Hospital-related infections, particularly from intensive care units, are brought about by the bacteria
Pseudomonas aeruginosa. Because it is resistant to drugs and may exist in hospitals, it can be hard to
kill off. Because of this, it creates a significant risk and challenge in hospital situations. The chemical
and genetic traits of Pseudomonas aeruginosa, most notably its beta-lactamase synthesis and antibiotic
resistance, have been part of several studies performed in the Al-Diwaniyah Province. One of the more
crucial phases when developing antimicrobial tactics is identifying the biological indicators associated
with antibiotic-resistant bacteria.
While Pseudomonas aeruginosa had been isolated from a number of clinics in past studies, here remains
not many assets available for its molecular characterization and resistance patterns. Finding out more on
a generalization of genes associated with resistance like GES, blaOXA-48, and SPM can help build
approaches for managing bacterial infections. Using traditional molecular techniques, this study sought
to obtain and determine Pseudomonas aeruginosa from medical samples, investigate its capacity to
resist drugs, and identify its presence of genes that create beta-lactamases. Findings: 15 out of 30 clinical
specimens (50%) had Pseudomonas aeruginosa confirmed. Burns (60%), wounds (26.6%), and urine
(13.4%) had the greatest isolation rates.
60% of the isolates produced beta-lactamase, suggesting that they were resistant to a lot of antibiotics
with the notable exception of tigecycline, as determined by the Rapid Iodine Assay and the Vitec II 2
system. The PCR method revealed that the 16S rRNA gene was found in all isolates. In addition, 20%
had the SPM gene, 40% had the blaGES gene, and 75% had the OXA-48 gene. This study is one of the
first to explain Pseudomonas aeruginosa’s molecules in the Diwaniyah Province focused on the genetic
factors that lead to its heightened resistance and production of beta-lactamases. we are consequences, To
avoid the development of drug-resistant Pseudomonas aeruginosa in healthcare environments, findings
show the urgent importance for ongoing surveillance, strict infection control procedures, and antibiotic
control programs.

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