Ali A. Obais¹, Maysaa S.M. Al-shukri², Abeer Fauzi M. Al-Rubaye³

College of Medical and Health Technique/ Department of Anesthesia/ Sawa University¹

Collage of Medicine/ Department of Microbiology/  Babylon University²

College of Science for women/ Department of Biology/ Babylon University³

Abstract

Objective :The present study was conducted to detect P gingivalis by culture and molecular method from clinical oral infection sample.  Method:  hundred fifty (150) paper point were collected from periodontitis patients who  attended to specific dental health center and  outpatient clinics of dentistry  in Al-Hillah city/ Iraq during the duration from (April 2022 to September 2022 ) . The age of patient (5– 72)years. The samples were collected by paper point  the paper point culture on blood agar and selective media(P. gingivalis agar (P.GING)) for isolation of P gingivalis then diagnosis conform by molecular method.  Results: The result showed that  out of 150 samples 15(10%) P.gingivalis isolated from this fifteen isolate only 7(4.66%) confirmed with molecular detection as  P.gingivalis. P.gingivalis isolated in high rate 6(4%) from age group (25-35) and 4(2.66) in the age group (15-25) while only 2 (1.33%) isolate obtained from the age group (45-55) and 3 (2%)  isolate from age group (55-70). Conclusion: quantitative evaluation, RT-PCR offers the advantage of eliminating false positives which could otherwise play abysmal role in conventional detection techniques.

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