Ali.H.Al-Basheer1 , Eyhab R.M.Al-Samawy2 , Tarteel Faisal Ghaze3

1College of Pharmacy , Sawa University, Iraq
2,3College of Medicine. Al- Muthanna University, Iraq

Abstract

Twenty birds were used to conduct the current study, aged 1-2 years, the Jejunum samples were collection
from September 2024 to February 2025, the birds collected at Al-Samawa abattoir and the study was
conducted in department of anatomy, histology in college of veterinary medicine university of Al
Muthanna.
The Jejunum wall consists from the four common known layers of a tube organ: tunica mucosa, tunica
submucosa, muscularis Externia and tunica serosa and adventitia.
Histochemical by applying three stains: PAS, AB(PH-2.5) PAS-AB [pH 1] stain. These staining
techniques were conducted to view the presence or absence of neutral mucins, acidic mucins and neutral
mucins with acidic mucins respectively. Both two types of cells of villi, which were the simple columnar
cells that reacted negatively with PAS technique and the goblet cells which reacted strongly with this
technique.
By using the alcian-Blue(PH-2.5) stain the goblet cells were revealed the strong reaction in both villi and
the crypt of lieberkühn . These reactions of AB stain indicate presence of acidic mucins in the villi and
crypts.
On using the combined PAS-AB (PH-1) for staining the tissues of Jejunum wall in guinea fowl, the
columnar epithelial cells reacted negatively, whereas the goblet cells reacted moderate with this stain,
indicating the presence of neutral and acidic muccopolysaccharide, the connective tissue of both lamina
propria, submucosa and serosa sustain negative reaction. The smooth muscle fibers of muscularis mucosa
and tunica muscularis afforded negative reaction with the PAS stain, AB and PAS + AB.

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