Anhar. A.Khinfoor1, Sabreen .H.Hussein2, Zainab .S. Daaboul Al-Hassani3

1Department of Surgery and Obstetrics,College of Veterinary Medicine,Al-Muthanna University,Al-Muthanna,
Al-Samawa 66001, Iraq Email: anhar.ahmed@mu.edu.iq
2Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, Al-Muthanna
University,Al-Muthanna, Al-Samawa 66001, Iraq Email: sabreen.hamed@mu.edu.iq
3Department of Pathological Analyses, College of Applied Medical Sciences,Al-Muthanna University,Al
Rumaytha 66002, Iraq Email: zainab.saad@mu.edu.iq

Abstarct

Background: The public health implications of burn injuries are substantial, and conventional burn
treatments can result in prolonged healing time and negative side effects. Recently, there has been
increased interest in using natural products and their active ingredients for use as alternative therapies for
wound care. The purpose of this investigation was to determine the use of a new topical ointment
composed of sesame oil and garlic extracts for treating second-degree burn injuries. Objective: The
effects of sesame/garlic ointment extract on wound healing will be tested by measuring the area of the
wound, the time required for the epithelial layer to heal, and the effects of the ointment on the appearance
of histopathological changes in the tissue of the rabbits.
Methods: A total of fifty New Zealand rabbits were randomly separated into two groups (n=25) as
follows: Group A (the control group; received standard petroleum jelly) and Group B (the treatment group;
received sesame and garlic ointment). Second-degree burn injuries were inflicted to the dorsum (back) of
each rabbit. The sesame and garlic ointment was created using cold-pressed sesame oil containing sesamol
and an alcoholic garlic extract (15% concentration containing allicin) blended with a base of petroleum
jelly. The ointment was applied to the wounds in both groups twice daily for 14 days, and measurements
of the area of each wound were recorded on days 0, 7, and 14. Tissue was also collected from the rabbits
on days 7 and 14 for histopathological evaluations.
Results: In the current study, the treatment of second-degree burns with sesame and garlic ointment
showed that treatment with ointment was statistically better at healing second-degree burns compared to
the control group. On day 7, the treated group exhibited good levels of angiogenesis (the production of
new blood vessels), abundant amounts of thickly deposited collagen fibers, and an infiltration of
macrophages into the wound site. By day 14, there were signs of nearly complete epithelialization of the
wound in the treated group, and there were continued signs of both good levels of angiogenesis and an
increase in the number of fibroblasts. On the other hand, the delayed healing response, combined with the
incomplete epithelialization, disorganized arrangement of collagen fibers, and large amounts of
inflammatory infiltrates on the 14th day, was observed in the control group.
Conclusion: The application of the sesame and garlic ointment extract produced marked wound healing
effects. In summary, the sesame and garlic ointment extract promotes faster epithelialization, improved
angiogenesis, and enhanced tissue remodeling. Additionally, these findings suggest that this product may
provide an effective natural therapy for the management of burn wounds, and that further clinical studies
should be conducted to confirm these observations.

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