A new spectrofluorimetric method for the determination of chloramphenicol in pharmaceutical preparations based on fluorescence quenching of methanol
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October 15, 2025Dheyaa Yahaia Alhameedi 1 , Ahmed Sapoori Sachit 2
1Department of Anesthesia Technologies, College of Health & Medical Technology, Sawa
University, Almuthana, Iraq
2Department of Radiology and Ultrasound Technologies, College of Health & Medical Technology,
Sawa University, Almuthana, Iraq
Abstract
A new, simple, rapid, sensitive, selective, and accurate method for the spectrofluorimetric determination of Chloramphenicol (CAP) in different pharmaceutical preparations. Chloramphenicol as active antibiotic is widely used in the treatment the diseases. The spectrofluorimetric method is based on the chloramphenicol affect the fluorescence quenching of methanol, methanol is fluorescence solvent with the wavelength at λex. 273 and λem. 322 nm. The working range is 1-10 µg mL-1. Limit of detection (LOD), and limit of quantification (LOQ) are 0.21 ppm, and 0.65 ppm, respectively, the recoveries range 99.8%-101.4%. The method was successfully applied to determine the (CAP) quantity in its pharmaceutical preparations (Eye drops, Ointments and Capsules).
