Saba Hameed Alshebani1 , Hiba Hameed Zidan2
1Department of Basic Sciences, college 0f Dentistry, University of Al-Qadisiya
2Department of clinical laboratory sciences, college 0f pharmacy, University of Al-Qadisiya
Abstract:
Recurrent pregnancy loss (RPL) is one of the most difficult problems in reproductive health since
effective treatments are rarely accessible and its causes are frequently unclear.To identify the independent
culture vaginal microbiota of recurrent aborted women by sequencing 16S ribosomal RNA
.Vagina
specimens were collected from recurrent abortion women . Multiplex PCR technique was performed
based 16S RNA gene to identify the uncultured bacteria. The DNA sequencing method was performed
using Sanger’s method and phylogeny for mutation analysis by using NCBI BLAST analysis.
Megasphera,
Atopobium, Mobiluncus, Erysipelothrix, Catibacterium, and Bifdobacterium were
diagnosed . This alignment study revealed the 16S ribosomal RNA gene’s substitution mutations and
nucleotide alignment similarities across isolates . Phylogenetic tree analysis of local isolates related to
NCBI-BLAST showed genetic relationship with some variation or subsituation of nitrogen bases of
target nucleotides. The maternal vaginal microbiome has an important role in the recurrent pregnant loss
etiology.
Keywords: Bioinformatics ; Vaginal Microbiota ; Recurrent Aborted Women; Multiplex PCR techniqu
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