Prevalence and Genetic Diversity of Coxsackievirus B in Iraqi Pediatric Patients with Type 1 Diabetes Mellitus
October 10, 2025Impact of Arginine, Taurine, and Tryptophan on Neutrophil Function in Renal Failure Patients in Al-Muthanna City
October 10, 2025Dheyaa Yahaia Alhameedi 1 , Ahmed Sapoori Sachit 2
1Department of Anesthesia Technologies, College of Health & Medical Technology, Sawa
University, Almuthana, Iraq
2Department of Radiology and Ultrasound Technologies, College of Health & Medical Technology,
Sawa University, Almuthana, Iraq
Abstract
A new, simple, rapid, sensitive, selective, and accurate method for the spectrofluorimetric determination
of Chloramphenicol (CAP) in different pharmaceutical preparations. Chloramphenicol as active antibiotic
is widely used in the treatment the diseases. The spectrofluorimetric method is based on the
chloramphenicol affect the fluorescence quenching of methanol, methanol is fluorescence solvent with
the wavelength at λex. 273 and λem. 322 nm. The working range is 1-10 µg mL-1. Limit of detection (LOD),
and limit of quantification (LOQ) are 0.21 ppm, and 0.65 ppm, respectively, the recoveries range 99.8%
101.4%. The method was successfully applied to determine the (CAP) quantity in its pharmaceutical
preparations (Eye drops, Ointments and Capsules).
Keywords: Chloramphenicol (CAP), fluorescence quenching, methanol, Pharmaceutical preparation.
